Viraphong LulitanondWasun ChantratitaKamthorn ThammaprasertKanchana NimmanahaemindaPornchai MatangkasombutChalobon YoosookMahidol UniversityFaculty of Assoc. Medical SciencesChiang Mai University2018-02-272018-02-271994-01-01Molecular and Cellular Probes. Vol.8, No.6 (1994), 441-447089085082-s2.0-0028566611https://repository.li.mahidol.ac.th/handle/20.500.14594/9534To study the presence of transforming sequence Bgl II N of HSV-2 in cervical cancer tissues, we developed the nested polymerase chain reaction (PCR) for detecting such a sequence in paraffinembedded cervical tissue sections. Samples derived from 46 patients with premalignant and malignant lesions were tested. The sequence was found in 20-25% of total cases tested but not observed in any of the normal healthy controls. This study also indicates that for the detection of HSV-2 Bgl II N sequence in cervical tissue, the nested PCR may be more reliable than the in situ hybridization method. © 1994 Academic Press.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1006/mcpr.1994.1063