Saovanee LeelayoovaChotechuang PanasopnkulPongsri TippawangkosolPanupong Sahaisook2024-02-132024-02-13200720072007Thesis (M.Sc. (Tropical Medicine))--Mahidol University, 2007https://repository.li.mahidol.ac.th/handle/20.500.14594/96712Tropical Medicine (Mahidol University 2007)Serodiagnosis of Brugian filariasis is not available in Thailand and a good test has not been demonstrated in the literature. Therefore, this study evaluated excretorysecretory antigens, either non-delipidized (NDLP) or delipidized (DLP) crude extracts from Dirofilaria immitis fresh and post-RPMI 1640 cultured adult worms, from which NDLP-fresh male and ether-DLP-fresh female crude extracts were subsequently selected by immunoblot test using pooled Brugian and negative sera to discriminate the unsatisfied extracts. IgG-ELISA analyzed male ES antigen (0.5 ug/ml), 1:800- diluted sera of Brugian filariasis (36 cases), other parasitic infections (181) and negative controls (54), and 1:8,000 dilution of IgG-conjugate. The test gave 100% sensitivity and 96.17% specificity at cut-off value 0.587. Only 8 cases of six parasitic infections (4.42%) and 1 negative control (1.85%) were false-positive. Under the above conditions, ES female antigen produced 100% sensitivity and 81.12% specificity at cut-off value 0.616. False positives were found in 37 cases of twenty parasitic infections (20.11%) and 8 negative controls (14.81%). For NDLP-fresh male antigen (2 ug/ml), serum dilution (1:1,600), and IgG-conjugate dilution (1:8,000), gave 88.89% sensitivity and 81.28% specificity, at cut-off value 0.233. The DLPfresh female antigen used the same conditions as the male crude antigen, except for IgG-conjugate at 1:16,000, resulting in 94.44% sensitivity and 76.17% specificity at cut-off value 0.164. Twenty parasitic infections showed false positives, 24.31% (44/181) and 30.94% (56/181) for crude male and female antigens, respectively. By immunoblot, only fresh male crude antigen showed an interesting glycoprotein band of 20.62 kDa, but it was inconsistent with Brugian filariasis sera and also cross-reacted with Bancroftian filariasis (5/5), gnathostomiasis (1/3), and angiostrongyliasis (1/3). It was concluded that ES male antigen can probably be used in the serodiagnosis of Brugian filariasis by IgG-ELISA, and male crude antigen may be used in detecting lymphatic filariasis by IgG-immunoblot. However, a purified antigen is needed for further study.xxiii, 128 leaves : ill.application/pdfengผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้าDirofilaria immitisFilariasis -- ThailandAntigensMaster ThesisMahidol University