Sarinya PrateepchanachaiWasina ThakhiewSakamon DevahastinSomchart SoponronnaritMahidol UniversityKing Mongkut s University of Technology ThonburiAcademy of Science2020-01-272020-01-272019-06-15International Journal of Biological Macromolecules. Vol.131, (2019), 589-60018790003014181302-s2.0-85063064710https://repository.li.mahidol.ac.th/handle/123456789/50149© 2019 Elsevier B.V. Glycerol, gelatin and CO 2 were used to prepare chitosan-based packaging films with improved mechanical properties and seal strength. Nuclear magnetic resonance spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy and dynamic mechanical analysis were conducted; the results were used to explain the mechanical and sealing properties data. Film color was also measured. Combined addition of glycerol and use of CO 2 to treat the film-forming solution led to decreased film crystallinity and glass transition temperature, resulting in improved film mechanical properties. Addition of glycerol and gelatin resulted in enhanced molecular interactions between chitosan-gelatin and improved sealability of the films. Films with the best mechanical and sealing properties were obtained from a solution with 25% (w/w) glycerol and treated with CO 2 and from that with 25% (w/w) glycerol and 10% (w/v) gelatin, respectively. Films with addition of both glycerol and gelatin exhibited lower lightness, but higher yellowness than the commercial stretch film.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyEconomics, Econometrics and FinanceEnergyArticleSCOPUS10.1016/j.ijbiomac.2019.03.067