Angela M. MinassianYrene ThemistocleousSarah E. SilkJordan R. BarrettAlison KempDoris QuinkertCarolyn M. NielsenNick J. EdwardsThomas A. RawlinsonFernando Ramos LopezWanlapa RoobsoongKatherine J.D. EllisJee Sun ChoEerik AuninThomas D. OttoAdam J. ReidFlorian A. BachGeneviève M.C. LabbéIan D. PoultonArianna MariniMarija ZaricMargaux MulatierRaquel Lopej RamonMegan BakerCelia H. MittonJason C. SousaNattawan RachaphaewChalermpon KumpitakNongnuj ManeechaiChayanut SuansomjitTianrat PiteekanMimi M. HouBaktash KhozoeeKirsty McHughDavid J. RobertsAlison M. LawrieAndrew M. BlagboroughFay L. NugentIona J. TaylorKimberly J. JohnsonPhilip J. SpenceJetsumon SattabongkotSumi BiswasJulian C. RaynerSimon J. DraperFaculty of Tropical Medicine, Mahidol UniversityCambridge Institute for Medical ResearchUniversity of CambridgeThe University of EdinburghUniversity of OxfordWalter Reed Army Institute of ResearchWellcome Sanger InstituteUniversity of Oxford Medical Sciences Division2022-08-042022-08-042021-12-08JCI Insight. Vol.6, No.23 (2021)237937082-s2.0-85120879529https://repository.li.mahidol.ac.th/handle/123456789/77407Controlled human malaria infection (CHMI) provides a highly informative means to investigate host-pathogen interactions and enable in vivo proof-of-concept efficacy testing of new drugs and vaccines. However, unlike Plasmodium falciparum, well-characterized P. vivax parasites that are safe and suitable for use in modern CHMI models are limited. Here, 2 healthy malaria-naive United Kingdom adults with universal donor blood group were safely infected with a clone of P. vivax from Thailand by mosquito-bite CHMI. Parasitemia developed in both volunteers, and prior to treatment, each volunteer donated blood to produce a cryopreserved stabilate of infected RBCs. Following stringent safety screening, the parasite stabilate from one of these donors (PvW1) was thawed and used to inoculate 6 healthy malaria-naive United Kingdom adults by blood-stage CHMI, at 3 different dilutions. Parasitemia developed in all volunteers, who were then successfully drug treated. PvW1 parasite DNA was isolated and sequenced to produce a high-quality genome assembly by using a hybrid assembly method. We analyzed leading vaccine candidate antigens and multigene families, including the vivax interspersed repeat (VIR) genes, of which we identified 1145 in the PvW1 genome. Our genomic analysis will guide future assessment of candidate vaccines and drugs, as well as experimental medicine studies.Mahidol UniversityMedicineArticleSCOPUS10.1172/jci.insight.152465