V. DesakornM. D. SmithA. L. WalshA. J.H. SimpsonD. SahassanandaA. RajanuwongV. WuthiekanunP. HoweB. J. AngusP. SuntharasamaiN. J. WhiteMahidol UniversitySappasitthiprasong HospitalNuffield Department of Clinical MedicineMusgrovc Park HospitalWellcome Trust Centre2018-09-072018-09-071999-01-01Journal of Clinical Microbiology. Vol.37, No.1 (1999), 117-121009511372-s2.0-0032898776https://repository.li.mahidol.ac.th/handle/123456789/25767Penicillium marneffei is a major cause of opportunistic infection in patients with AIDS in north and north-eastern Thailand. A method for the quantitation of P. marneffei antigen in urine was developed by using fluorescein isothiocyanate-labelled purified rabbit hyperimmune immunoglobulin G in an enzyme-linked immunosorbent assay. This method was evaluated with 33 patients with culture-proven penicilliosis and 300 controls (52 healthy subjects, 248 hospitalized patients without penicilliosis) from the same area in which penicilliosis is endemic. Urinary antigen was found in all 33 (100%) patients with penicilliosis, with a median titer of 1:20,480. With undiluted samples, 67 (27%) of 248 hospital patients and 3 (6%) of 52 healthy controls were reactive. At a cutoff titer of 1:40, the urine antigen detection assay had a diagnostic sensitivity of 97% and specificity of 98% (positive predictive value, 84%; negative predictive value, 99.7%). This test offers a valuable and rapid method for the diagnosis of penicilliosis in patients with AIDS and could be a useful addition to conventional diagnostic methods in areas in which penicilliosis is endemic.Mahidol UniversityMedicineArticleSCOPUS