Suchart ChanamaChanwit SuriyachadkunManee ChanamaChulalongkorn UniversityMahidol UniversityThailand National Center for Genetic Engineering and Biotechnology2022-08-042022-08-042021-01-01International Journal of Systematic and Evolutionary Microbiology. Vol.71, No.3 (2021)14665034146650262-s2.0-85103318607https://repository.li.mahidol.ac.th/handle/123456789/75792A novel actinomycete, strain SMC 257T, was isolated from a soil sample collected from mountain forest, Nan Province, Thailand. Strain SMC 257T formed tightly closed spiral spore chains on aerial mycelia. A polyphasic approach was used for the taxonomic study of this strain. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMC 257T belonged to the genus Nonomuraea, and the closest phylogenetically related species were Nonomuraea roseoviolacea subsp. carminata JCM 9946T (98.9% 16S rRNA gene sequence similarity), Nonomuraea rhodomycinica TBRC 6557T (98.4%), and Nonomuraea roseo-violacea subsp. roseoviolacea JCM 3145T (98.3%). Genome sequencing revealed a genome size of 9.76 Mbp and a G+C content of 72.3 mol%. The genome average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values that distin-guished this novel strain from its closest related species were species boundary of 95–96% and 70%, respectively. The cell wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars were glucose, ribose, madurose and mannose. The major menaquinone was MK-9(H4 ). The polar lipid profile consisted of phosphatidylethanolamine, hydroxyphosphatidylethan-olamine, lysophosphatidylethanolamine, diphosphatidylglycerol, N-phosphatidylglycerol, phosphatidylinositol and phosphati-dylinositol mannosides. The predominant cellular fatty acids were C17:0 10-methyl and iso-C16:0 . Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, strain SMC 257T is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea montanisoli is proposed. The type strain is SMC 257T (=TBRC 13065T=NBRC 114772T).Mahidol UniversityAgricultural and Biological SciencesImmunology and MicrobiologyArticleSCOPUS10.1099/ijsem.0.004695