Chiangnoon R.Karawak P.Eamsiri J.Thamrongsiripak N.Uttayarat P.Angkhasirisap W.Ampawong S.Mahidol University2023-06-182023-06-182022-01-01BMEiCON 2022 - 14th Biomedical Engineering International Conference (2022)https://repository.li.mahidol.ac.th/handle/123456789/84313Hydrogels have emerged as a new class of materials that are capable of providing appropriate moist environment for wound healing. In this report, gamma irradiation was utilized to prepare flexible, self-Adhesive, and biocompatible hydrogel sheets from polyvinyl alcohol (PVA) as wound dressings. The PVA concentrations ranged from 6-10 % (w/v) and gamma doses from 10-80 kGy were first varied to optimize the formation of crosslinked, three-dimensional structure of hydrogels. Based on swelling behavior, the 10% (w/v) PVA solution crosslinked at the dose of 40 kGy was selected to fabricate prototypic hydrogel sheets that could conform over skin contour. The extraction-based MTT assay showed that the hydrogel sheets were cytocompatible with L929 cells. When applied on full-Thickness defects in rat model, the hydrogel sheets could facilitate re-epithelization to complete wound closure. These results demonstrate that our PVA hydrogel sheets developed by gamma irradiation can serve as a suitable candidate for wound dressings.Computer ScienceConference PaperSCOPUS10.1109/BMEiCON56653.2022.100120792-s2.0-85147246983