Chotechana WilailuckanaChanwit TribuddharatChuntima TiensasitornPintip PongpechPenphun NaennaSiriporn RugdeekhaChertsak DhiraputraSomwang DanchaivijitrKhon Kaen UniversityMahidol UniversityChulalongkorn University2018-08-202018-08-202006-03-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.2 (2006), 327-334012515622-s2.0-33746050315https://repository.li.mahidol.ac.th/handle/123456789/23815Discriminatory powers of various molecular techniques were evaluated for typing of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Siriraj Hospital, Bangkok, Thailand. Thirty MRSA isolates were randomly selected in this study. They were characterized by pulsed-field gel electrophoresis, ClaI-mecA and ClaI-Tn554 polymorphisms, ribotyping, and PCR-based methods including SCCmec typing, spa and coa gene polymorphism, and repeat units in hypervariable region downstream of mecA. Individual molecular typing technique distinguished those MRSA isolates into 2 to 5 types. Eleven genetic backgrounds of MRSA isolates were elucidated by combination of typing methods with trimethoprim/sulfamethoxazole (TMP/SXT) susceptibility. Combination of all typing methods including TMP/SXT susceptibility yielded a discriminatory index of 0.94. Combination of PCR-based methods and TMP/SXT susceptibility, with the discriminatory index of 0.89, is a practical typing approach suitable for rapid epidemiological investigation of MRSA isolates in a hospital setting.Mahidol UniversityMedicineArticleSCOPUS