Naoya KataokaAlisa S. VangnaiThunyarat PongtharangkulTakahisa TajimaToshiharu YakushiKazunobu MatsushitaJunichi KatoYamaguchi UniversityChulalongkorn UniversityMahidol UniversityHiroshima University2018-11-232018-11-232015-06-01Journal of Biotechnology. Vol.204, (2015), 25-3218734863016816562-s2.0-84927747893https://repository.li.mahidol.ac.th/handle/123456789/35447© 2015 Elsevier B.V. 1-Butanol is an important industrial platform chemical and an advanced biofuel. While various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. Here, we constructed a CoA-dependent 1-butanol synthetic pathway that functions under aerobic conditions in Escherichia coli, by expanding the previously reported (R)-1,3-butanediol synthetic pathway. The pathway consists of phaA (acetyltransferase) and phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, phaJ ((R)-specific enoyl-CoA hydratase) from Aeromonas caviae, ter (trans-enoyl-CoA reductase) from Treponema denticola, bld (butylraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and inherent alcohol dehydrogenase(s) from E. coli. To evaluate the potential of this pathway for 1-butanol production, culture conditions, including volumetric oxygen transfer coefficient (k<inf>L</inf>a) and pH were optimized in a mini-jar fermenter. Under optimal conditions, 1-butanol was produced at a concentration of up to 8.60 gL^-1 after 46h of fed-batch cultivation.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringImmunology and MicrobiologyArticleSCOPUS10.1016/j.jbiotec.2015.03.027