Patima PhainuphongVatcharin RukachaisirikulSouwalak PhongpaichitJariya SakayarojPhongthon KanjanasiriratSuparerk BorwornpinyoNattaphong AkrimajirachooteChantapol YimnualChatchai MuanprasatWalailak UniversityMahidol UniversityPrince of Songkla University2019-08-232019-08-232018-09-27Tetrahedron. Vol.74, No.39 (2018), 5691-569914645416004040202-s2.0-85051529528https://repository.li.mahidol.ac.th/handle/20.500.14594/45049© 2018 Elsevier Ltd Three new depsides, aspergisides A-C, and one new depsidone, aspergisidone, were isolated from the soil-derived fungus Aspergillus unguis PSU-RSPG204 together with one phthalide derivative and 11 depsidones including emeguisin A, aspersidone and folipastatin. The structures were determined by spectroscopic evidence. Aspersidone and emeguisin A showed remarkably antibacterial activity against Staphylococcus aureus and methicillin-resistance S. aureus with equal MIC values of 0.5 μg/mL. Emeguisin A, which displayed the highest activity with 87.06% inhibition of human colon carcinoma (HCT-116) cell viability, decreased numbers of live cells/numbers of dead cells in a dose-dependent manner with the IC50 values of 34.8–84.7 μM in a 3D culture model depending on durations of incubation. In addition, folipastatin dose-dependently inhibited forskolin-stimulated chloride secretion in human intestinal epithelial (T84) cells with an IC50 value of 0.5 μM. These depsidones were considered to be inactive against noncancerous Vero Cells with the IC50 values of >10 μM.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1016/j.tet.2018.07.059