A. WisitsoraatS. PakapongpanC. SriprachuabwongD. PhokharatkulP. SritongkhamT. LomasA. TuantranontThailand National Electronics and Computer Technology CenterMahidol University2018-10-192018-10-192013-01-01Journal of Electroanalytical Chemistry. Vol.704, (2013), 208-213157266572-s2.0-84881277565https://repository.li.mahidol.ac.th/handle/123456789/31497In this work, a highly sensitive enzyme-based biosensor is developed based on graphene-poly(3,4-ethylenedioxythiophene):polystyrene sulfonic acid (GP-PEDOT:PSS) modified screen printed carbon electrode (SPCE) for electrochemical detection of glucose. GP-PEDOT:PSS nanocomposite synthesized by one-step electrolytic exfoliation is drop-coated on SPCE and glucose oxidase (GOD) enzyme is then immobilized on GP-PEDOT:PSS by glutaraldehyde cross linking. Electron microscope characterization confirms the presence of multi-layer graphene sheets and the appearance of smooth and long microstripe structure of GOD on the electrode surface. Direct electrochemistry of GOD at the GP/PEDOT:PSS electrode is observed by cyclic voltammetry and graphene plays the main role in a quasi-reversible redox process. GOD/GP-PEDOT:PSS electrode exhibits a high amperometric sensitivity of 7.23 μA/mM, which is about 13 times higher than that of GOD/PEDOT:PSS but has a relatively narrow linear dynamic range of ∼20-900 μM. Moreover, GOD/GP-PEDOT:PSS offers a very low detection limits (3S/N) of ∼0.3 μM which is better or comparable to other recent reports of highly sensitive glucose biosensors. Furthermore, the sensor has good stability with only 30% loss of enzyme activity after 30 days. © 2013 Elsevier B.V. All rights reserved.Mahidol UniversityChemical EngineeringChemistryArticleSCOPUS10.1016/j.jelechem.2013.07.012