Praphathip EamsobhanaHoi Sen YongJoon Wah MakDorn WattanakulpanichMahidol UniversityUniversity of MalayaInstitute for Medical Research Kuala Lumpur2018-07-042018-07-041997-01-01International Journal of Infectious Diseases. Vol.2, No.1 (1997), 34-36120197122-s2.0-0030719410https://repository.li.mahidol.ac.th/handle/20.500.14594/18204Objective: To confirm antigenic differences between Parastrongylus cantonensis and Parastrongylus malaysiensis. Methods: Sodium dodecyl sulfate- polyacrylamide gel electrophoresis and immunoblotting were conducted. Results: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the somatic extracts of R cantonensis and P. malaysiensis adult worms were highly complex with no apparent differences in the gel patterns between the two species. Immunoblot analysis using AW-3C2, a monoclonal antibody produced against the adult worm of P. cantonensis, showed variable staining smears in Parastrongylus adult worm antigens. With male worm antigens, a distinct antigenic band with apparent molecular weight of 205 kDa in P. cantonensis and one of 180 kDa in P. malaysiensis were observed. Conclusion: Antigenic differences exist between R cantonensis and P. malaysiensis among proteins with apparently identical molecular weight.Mahidol UniversityMedicineArticleSCOPUS10.1016/S1201-9712(97)90009-6