Thirayudh GlinsukonChaivat ToskulkaoPunya TemcharoenChulee Ubolsakka2025-02-032025-02-03199220251992Thesis (M.Sc. (Physiology))--Mahidol University, 1992https://repository.li.mahidol.ac.th/handle/20.500.14594/103255Physiology (Mahidol University 1992)Since endurance training is considered as a strategy for prevention or reduction in the severity of disease in many organs and also the high risk in liver damage as high exposure to xenobiotic now a days. Therefore, the effect of endurance training pretreatment on severity of aflatoxin Bl (AFB1) hepatotoxicity and the activities of some drug metabolizing and detoxifyiny system were examined in adult male rats. In the study of endurance training effect, the rats were subjected to swimming with 1% BW resistance for 30 min, 5 days/week for 14 weeks. Endurance training induced high physical fitness as shown by reduction in final body weight (11.73%), reduction in resting heart rate (13.88%), increase in relative heart weight (22.61%), increase in the activities of succinate dehydrogenase (49.11%) and citrate synthase (152.25%) in gastrocnemius muscle. It also had an activating effect on the activities of aniline hydroxylase and p-nitroanisole-O-demethylase in microsome of liver as shown by 87% and 75.6% increase in both enzymes, respectively. The activation is reversible in 60-72 hours after training. But this did not occur to hepatic glutathione-S-transferase enzyme. Lipid peroxide and glutathione (GSH) content of liver and liver cell integrity were not affected. Water immersion had not any significant influence. In the study of severity of hepatotoxicity, the rats were pretreated with similar endurance training program followed by single i.p. injection of AFB1 in a dose of 2 mg/kg BW. The hepatotoxicity, some drug metabolizing and GSH detoxifying system and histopathological changes were investigated at 0, 12 and 24 hours after AFB1 administration. It has shown more increase in the activities of serum alanine aminotransferase (8 folds), aspartate aminotransferase (1.8 folds) and severe histopathological changes at 24 hours in trained group after AFB1 administration. These results suggested that endurance training pretreatment enhanced the severity of hepatocellular necrosis at 24 hours after AFB1 administration. More increased in hydroxylation and relative lower detoxification via GSH conjugation at the initial and 12-24 hours after AFB1 administration were likely to be the enhancing factor. Lipid peroxide contributed to AFB1 hepatotoxicity but it was not the enhancing factor in this case. However, the activation on the-phase I metabolism by training may lead to less hepatotoxicity if lower dose of AFB1 is administered. Further studies are warranted.xv, 178 leaves : ill.application/pdfengผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้าAflatoxin B1 -- toxicityExcercisePhysical fitnessHepatotoxicologyMaster ThesisMahidol University