PETER S. AKAISWANGJAI PUNGPAKVIROJ KITIKOONDANAI BUNNAGA. DEAN BEFUSUniversity of CalgaryMahidol UniversityUniversity of Alberta2018-02-272018-02-271994-01-01Parasite Immunology. Vol.16, No.6 (1994), 279-28813653024014198382-s2.0-0028023315https://repository.li.mahidol.ac.th/handle/20.500.14594/9585Chronic infections with the liver flukes Opisthorchis viverrini and Clonorchis sinensis affect over 30 million people in southeastern Asia. With ongoing exposure, reinfection readily occurs following curative treatment and cumulative infections result in significant morbidity and a predisposition to cholangiocarcinoma. Though protective immunity has never been described in human opisthorchiasis, heterogeneity in worm burden occurs and a small number of exposed residents of endemic areas remain apparently uninfected. To explore the nature of this heterogeneity, we compared levels of serum antibody (Ab) to O. viverrini measured by an enzyme‐linked immunosor‐bent assay in 83 stool egg‐positive and 49 stool egg‐negative residents of an O. viverrini‐endemic area in Thailand. Compared to the egg‐positive residents, the egg‐negative group had significantly higher levels of immunoglobulin (Ig)G, IgA and IgM to adult worm homogenate (AWH) and total Ab to metacercaria homogenate (MH). Furthermore, immunoblot analyses revealed that a significantly higher proportion of sera from the egg‐negative residents had IgA reactivity against a 38‐k Da AWH antigen and IgM reactivity against carbohydrate epitopes of a 42‐k Da AWH glycoprotein antigen. These findings support a hypothesis that the egg‐negative group includes individuals who may be immunologically resistant to this usually chronic infection. Copyright © 1994, Wiley Blackwell. All rights reservedMahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1111/j.1365-3024.1994.tb00350.x