Rujirat HatrongjitAnusak KerdsinDan TakeuchiThidathip WongsurawatPiroon JenjaroenpunPeechanika ChopjittParichart BoueroyYukihiro AkedaShigeyuki HamadaKasetsart University, Chalermphrakiat Sakon Nakhon Province CampusResearch Institute for Microbial DiseasesUniversity of Arkansas for Medical SciencesOsaka UniversityFaculty of Medicine, Siriraj Hospital, Mahidol University2021-02-032021-02-032020-12-01Pathogens. Vol.9, No.12 (2020), 1-13207608172-s2.0-85098523752https://repository.li.mahidol.ac.th/handle/20.500.14594/60878© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The resistance of Gram-negative bacteria to colistin, mediated by plasmid-borne mcr genes, is an emerging public health concern. The complete genome sequence (4.55 Mb) of a clinical isolate of Aeromonas veronii biovar veronii obtained from a patient with septicemia was determined using short-read and long-read platforms. This isolate (C198) was found to harbor a novel mcr-3 gene, designated mcr-3.41. Isolate C198 revealed adjacent mcr-3.41 and mcr-3-like genes. It contained one chromosome and two plasmids, both of which encoded a RepB replication protein. Other antimicrobial resistance genes, including blacphA3, blaOXA-12, tetA, rsmA, and adeF, were also present. Isolate C198 was resistant to amoxicillin–clavulanate, ampicillin–sulbactam and tetracycline, and showed intermediate resistance to trimethoprim–sulfamethoxazole. The isolate was susceptible to piperacillin–tazobactam, carbapenem, third-generation cephalosporins, fluoroquinolones, chloramphenicol, and aminoglycosides. Putative virulence genes in the C198 genome encoded type II, III, and VI secretion systems; type IV Aeromonas pili; and type I fimbria, flagella, hemagglutinin, aerolysin, and hemolysins. Multilocus sequence typing revealed a novel sequence type (ST), ST720 for C198. Phylogenetic analysis of the single nucleotide polymorphisms in C198 demonstrated that the strain was closely related to A. veronii 17ISAe. The present study provides insights into the genomic characteristics of human A. veronii isolates.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.3390/pathogens9121031