Wannaporn IttiprasertPiyarat ButrapornViroj KitikoonKosoom KlongkamnuankarnKhamlien PholsenaViengxay VanisavethYuwaporn SakolvareeManas Chongsa-NguanPramuan TapchaisriYuvadee MahakunkijcharoenHisao KurazonoHideo HayashiWanpen ChaicumpaMahidol UniversityInstitute of Malaria, Parasitic Diseases and EntomologyUniversity of Tsukuba2018-09-072018-09-072000-09-01Parasitology International. Vol.49, No.3 (2000), 209-218138357692-s2.0-0034284445https://repository.li.mahidol.ac.th/handle/20.500.14594/25975An indirect (plate) ELISA and, a more convenient version, a dot-blot (membrane) ELISA have been developed using haemocyanin of a mollusk, Megathura crenulata, i.e. keyhole limpet haemocyanin (KLH) and purified, specific antigen of Trichinella spiralis (APTsAg) obtained from a monoclonal antibody-affinity column chromatography, for differential diagnosis of schistosomiasis mekongi and trichinellosis. Serum samples of patients with parasitologically confirmed trichinellosis were reactive to both antigens in both versions of ELISA while sera of patients with schistosomiasis mekongi were positive only to the KLH. Both ELISA were negative when used to test sera of normal controls and patients with gnathostomiasis, paragonimiasis and opisthorchiasis. (C) 2000 Elsevier Science Ireland Ltd.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/S1383-5769(00)00046-5