Theerapong KrajaejunPiriyaporn ChongtrakoolKanong AngkananukulTristan T. BrandhorstMahidol UniversityUniversity of Wisconsin School of Medicine and Public Health2018-09-242018-09-242010-11-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.6 (2010), 1462-1466012515622-s2.0-79952610173https://repository.li.mahidol.ac.th/handle/123456789/29480Pythium insidiosum causes a potentially life-threatening infectious disease called pythiosis. An early, accurate diagnosis is important, since prompt treatment leads to a better prognosis. Unsuccessful attempts to isolate the organism have been associated with specimens subjected to lower temperatures. We analyzed growth of P. insidiosum at various temperatures. Culture at low (8°C) and high (42°C) temperatures resulted in death or inhibited growth of the organism. Culture under optimal temperatures (28 and 32°C) was important for successful isolation of P. insidiosum.Mahidol UniversityMedicineArticleSCOPUS