Chutatip Siripanthจุฑาทิพ ศิริพันธุ์Benjanee Punpoowongเบญจนี พันธ์ภูวงศ์Pornsawan Ammarapalพรสวรรค์ อัมระปาลNiramol ThimaMahidol University. Faculty of Tropical Medicine. Department of ProtozoologyMahidol University. Faculty of Tropical Medicine. Department of Tropical PathologyMahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology2016-04-042021-09-022016-04-042021-09-022016-04-042004https://repository.li.mahidol.ac.th/handle/123456789/63429Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.224.Isospora belli, Cryptosporidium parvum sporozoites and Microsporidial spores were infected into culture cells. The developmental stages of these intracellular protozoa were described after the fresh observation and staining of infected cells on cover glasses. Paraffin tissue section was performed and compared to cover glass staining. Fresh observation was more advantages for the observation of the movement of I. belli merozoites but the development of merogony of Cryptosporidium could not be notified by fresh observation. The merogony stages of I. belli from paraffin tissue sections were not clear, compared to cover glass staining but the trophozoites of Cryptosporidium were clearly demonstrated by paraffin tissue sections. The typical characters of belt-like stripes of Microsporidial spores from culture were clearly observed from cover glass staining. In conclusion, cover glass staining could be used for an alternative method for the detection of intracellular protozoa from cultures. However, the properstaining methodsshould be selected for each protozoan parasite.engMahidol UniversityParasiteProceeding Poster