Sontisiri P.Promrug D.Arthan D.Choengchan N.Thongyoo P.Mahidol University2025-01-232025-01-232025-02-05Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Vol.326 (2025)13861425https://repository.li.mahidol.ac.th/handle/20.500.14594/102756A new “OFF–ON” coumarin-based fluorescent probe for H2S detection was designed and successfully developed through O-sulfonylation between a dabsyl quencher and 7-hydroxy-4-methylcoumarin as a fluorescent reporter, based on a FRET approach. This H2S responsive probe, utilizing H2S assisted thiolysis of a sulfonate ester as the sensing strategy, demonstrated excellent performance towards H2S with a limit of detection (LoD) of 1.64 µM, along with superb selectivity, good stability and high specificity towards H2S without interference from other biomarkers and analytes. Moreover, dabsyl-7-hydroxy-4-methylcoumarin (dabsylcoumarin) is capable of permeating the cell membrane and effectively visualizing the level of H2S in the living HeLa cells without cytotoxicity.ChemistryPhysics and AstronomyArticleSCOPUS10.1016/j.saa.2024.1251702-s2.0-8520494947239342725