Skorn MongkolsukSiritida RabibhadanaPaiboon VattanaviboonSuvit LoprasertChulabhorn Research InstituteMahidol University2018-02-272018-02-271994-05-27Gene. Vol.143, No.1 (1994), 145-146037811192-s2.0-0028260594https://repository.li.mahidol.ac.th/handle/123456789/9511We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the λ, ficI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequence which permitted mobilization of the plasmid into a wide variety of Gram - bacteria. © 1994.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/0378-1119(94)90620-3