Siroj JitprasutwitWisansanee ThaewpiaVeerachat MuangsombutAroonlug LulitanondChanvit LeelayuwatGanjana LertmemongkolchaiSunee KorbsrisateMahidol UniversityKhon Kaen Regional HospitalKhon Kaen University2018-09-242018-09-242010-09-03Journal of Microbiology. Vol.48, No.4 (2010), 526-53212258873122588732-s2.0-77956100328https://repository.li.mahidol.ac.th/handle/20.500.14594/29193Burkholderia thailandensis is a close relative of Burkholderia pseudomallei. These organisms are very similar, but B. thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis of 14 B. thailandensis isolates revealed variation in the regions coding for the type III secreted BipD protein. The degree of B. thailandensis BipD sequence variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B. thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant of culture medium unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce the ability of this bacterium to invade human respiratory epithelial cells A549. The identification of an environmental stimulus that increases the invasion capability of B. thailandensis invasion is of value for those who would like to use this bacterium as a model to study B. pseudomallei virulence. © 2010 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1007/s12275-010-0078-x