Paisarn JittorntrumSaowanee KajanachumpolDuangkamol ViroonudompholPrapin WilairatMahidol UniversityNational Institute of Metrology (Thailand)2018-11-092018-11-092014-02-11Chiang Mai Journal of Science. Vol.41, No.1 (2014), 156-165012525262-s2.0-84893496358https://repository.li.mahidol.ac.th/handle/20.500.14594/33303Tranexamic acid (TA) is a synthetic lysine analog used for the management of bleeding disorders. In this study, we developed and validated a method for the determination of TA in human serum by liquid chromatography with Q-trap mass spectrometer. Serum sample (100 μL) was deproteinated with perchloric acid, and after pH adjustment, chromatographic separation was performed on a C18 column and isocratically eluted using a mobile phase consisting of ammonium acetate buffer (pH 3.8) /acetonitrile (95:5, v/v) at a flow rate of 200 μL /min. The total run time was 5 minutes. Detection and quantitation were performed with the mass spectrometer using multiple reaction monitoring mode with the ion transition m/z 158.1 to m/z 95.1 for TA and m/z 144.0 to m/z 81.1 for the internal standard (cis-4-aminocyclohexanecarboxylic acid). The results were linear over the concentration range of 0.1-100 μg/mL of TA, with limit of quantitation of 0.03 μg/mL. The intra-day and inter-day assay coefficient of variations for serum were less than 1.8% and 2.1%, respectively, and the recovery of added standard TA was 92.5 to 99.3%. In conclusion; a simple and sensitive LC-MS/MS method has been developed for the determination of TA in human serum. The method showed excellent linearity, sensitivity, recovery and precision. This method is suitable for clinical pharmacokinetic studies.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryMaterials ScienceMathematicsPhysics and AstronomyArticleSCOPUS