Skorn MongkolsukAmaret BhumiratanaSiritida Rabibhadana2024-06-272024-06-27199119912024Thesis (M.Sc. (Microbiology))--Mahidol University, 1991https://repository.li.mahidol.ac.th/handle/20.500.14594/99057Microbiology (Mahidol University 1991)RecA-like gene of Xanthomonas oryzae isolate 8707 chromosomal DNA have been cloned by using rationale of complementation E. coli recA-mutation and selected for mitomycin C resistant phenotype. Four transformants from six thousand colonies were retransformed into E. coli DH5a, HB101 and BW368 and checked for MMS and mitomycin C resistant. Result from Southern blot analysis of one of these transformants named pSM-A1 confirmed that the insert portion of this clone derived from Xanthomonas oryzae isolate 8707 chromosomal DNA. Significantly, from immunological analysis antiserum raised against E. coli RecA protein cross-reacted with Xanthomonas phaseolin, Xanthomonas oryzae 8707, Xanthonmonas campestris pv campestris. Furthermore, it also cross-reacted with cell lysate from E. coli BW368 harboring pSM-A1 but it did not react with cell lysate from E. coli BW368 RecA protein, A single band detected from pSM A1 cell lysate with an apparent molecular weight nearly equal to a single band detected from Xanthomonas oryzae isolate 8707 cell lysate and a protein band molecular weight 40,000 E. coli protein, These results indicated that pSM-Al clone contains Xanthomonas oryzae isolate 8707 recA-like gene.v, 85 leaves : ill.application/pdfengผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้าGenetic complementation testXanthomonasMaster ThesisMahidol University