Dhammika Leshan WannigamaCameron HurstParichart HongsingLachlan PearsonThammakorn SaethangNaphat ChantaravisootUthaibhorn Singkham-InSirirat Luk-InRobin James StorerTanittha ChatsuwanUniversity of Western AustraliaChulalongkorn UniversityQIMR Berghofer Medical Research InstituteKasetsart UniversityMae Fah Luang UniversityMahidol UniversityThe Westmead Institute for Medical Research2020-05-052020-05-052020-03-13Annals of Clinical Microbiology and Antimicrobials. Vol.19, No.1 (2020)147607112-s2.0-85081976448https://repository.li.mahidol.ac.th/handle/20.500.14594/54619© 2020 The Author(s). Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.Mahidol UniversityMedicineArticleSCOPUS10.1186/s12941-020-00350-6