Sirithip ChuaijitWorawit BoonyatistanPichsinee BoonchuayChanatip MetheetrairutWichit SuthammarakFaculty of Medicine, Siriraj Hospital, Mahidol University2020-01-272020-01-272019-05-01Mitochondrion. Vol.46, (2019), 91-9618728278156772492-s2.0-85043998891https://repository.li.mahidol.ac.th/handle/20.500.14594/50186© 2018 Elsevier B.V. and Mitochondria Research Society Assembly of complex I of the mitochondrial respiratory chain (MRC) requires not only structural subunits for electron transport, but also assembly factors. In the nematode Caenorhabditis elegans, NUAF-1 and NUAF-3 are the only two assembly factors that have been characterized. In this study, we identify ACDH-12 as an assembly factor of the respiratory complex I. We demonstrate for the first time that a deficiency of ACDH-12 affects the formation and function of complex I. RNAi knockdown of acdh-12 also shortens lifespan and decreases fecundity. Although ACDH-12 has long been recognized as a very long-chain acyl-CoA dehydrogenase (VLCAD), the knockdown nematodes did not exhibit any change in body fat content. We suggested that in Caenorhabditis elegans, ACDH-12 is required for the assembly of the respiratory complex I, but may not be crucial to fatty acid oxidation. Interestingly, sequence analysis shows high homology between ACDH-12 and the human ACAD9, a protein that has initially been identified as a VLCAD, but later found to also be involved in the assembly of complex I in human.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.mito.2018.02.010