Victoria R. PolonisMark S. De SouzaJanice M. DardenSomsak ChantakulkijThippawan ChuenchitraSorachai NitayaphanArthur E. BrownMerlin L. RobbDeborah L. BirxArmed Forces Research Institute of Medical Sciences, ThailandMahidol UniversityHJFWalter Reed Army Institute of Research2018-07-242018-07-242003-08-01Journal of Virology. Vol.77, No.15 (2003), 8570-85760022538X2-s2.0-0038107685https://repository.li.mahidol.ac.th/handle/123456789/20891A number of human immunodeficiency virus type 1 (HIV-1) non-B-subtype products have been developed for present or future vaccine trials; in Thailand, several studies using subtype B and/or CRF01_AE vaccines have been conducted. To better characterize the biologic properties of these subtypes, 70 HIV-1 subtype B and E isolates were phenotyped as syncytium-inducing (SI) or non-syncytium-inducing (NSI) isolates and assessed for sensitivity to neutralizing antibody (NAb). A significantly higher number of NSI subtype E viruses were neutralization sensitive than SI subtype E viruses (P = 0.009), while no association between viral phenotype and sensitivity to NAb was observed for subtype B (P = 0.856), suggesting a difference in the neutralization patterns of subtypes B and E. Strikingly, concurrent CD4 T-cell numbers were significantly lower for subtype E-infected patients whose isolates were more resistant to NAb, both for the overall study group (P < 0.001) as well as for the 22 patients with NSI isolates (P = 0.013). Characterization of the evolution of biologic properties of both B and non-B HIV-1 subtypes will provide a clearer understanding of the repertoire of antibodies that must be elicited for a vaccine to be effective against all phenotypes and subtypes.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1128/JVI.77.15.8570-8576.2003