Sureewan SittijundaAlissara ReungsangFaculty of Environment and Resource Studies, Mahidol UniversityKhon Kaen University2019-08-232019-08-232018-01-01Waste and Biomass Valorization. (2018)1877265X187726412-s2.0-85058639358https://repository.li.mahidol.ac.th/handle/20.500.14594/45778© 2018, Springer Nature B.V. The optimization of factors affecting methane production from the co-digestion of algal biomass with crude glycerol was investigated using response surface methodology with a central composite design. The investigated parameters included algal biomass, crude glycerol and inoculum concentration. The inoculum and algal biomass concentration had a significant individual effect on the methane production (p ≤ 0.05). The interactive effect on methane production was found to be between algal biomass and crude glycerol (p ≤ 0.05). The optimal conditions were 20.02 g-VS/L of crude glycerol, 9.76 g-VS/L of inoculum concentration and 5.50 g-VS/L of algal biomass. Under optimum conditions, the maximum methane production of 58.88 mL-CH4/L was obtained. The difference between the observed methane production (58.88 mL-CH4/L) and the predicted methane (68.94 mL-CH4/L) was 14.59%. Under the optimum conditions, the energy production of 92.47 J/g-VSadded was obtained. The polymerase chain reaction-denaturing gradient gel electrophoresis analysis indicated that the methane producers present in the fermentation broth were Methanosarcina sp., Methanoregula sp., Methanospirillum sp. and Methanoculleus sp.Mahidol UniversityEnergyEnvironmental ScienceSCOPUS10.1007/s12649-018-0542-0