S. AngsubhakornJ. B. MoeN. J. MarchetteJ. R. LatendresseN. E. PalumboS. YoksanN. BhamarapravatiMahidol UniversityDivision of PathologyDepartment of Tropical Medicine and Medical Microbiology HonoluluWalter Reed Army Institute of ResearchUniversity of Hawaii at ManoaFaculty of Medicine, Ramathibodi Hospital, Mahidol University2018-06-142018-06-141987-01-01Journal of Virological Methods. Vol.18, No.1 (1987), 13-24016609342-s2.0-0023626858https://repository.li.mahidol.ac.th/handle/20.500.14594/15362The results of a comparative study of neurovirulence of dengue type 1 virus in two species of Old World monkeys, viz. rhesus monkeys (Macaco, mulatta) and cynomolgus monkeys (Macaco fascicularis) are reported. In the present study, parental dengue type 1 (16007) and its vaccine viruses were tested by intrathalamic, intramuscular and intraspinal injections in these two species of monkey. Both species of monkeys inoculated with parental dengue type 1 virus developed neurovi-rulence-type lesions which were graded as minimal (V-1) and occasionally mild (V-2, in cynomolgus monkeys) in severity. The antibody response to either parental or vaccine virus was slightly less in rhesus monkeys than in cynomolgus inoculated with these strains. This comparative study possibly establishes the cynomolgus monkey as a suitable test model to replace the rhesus monkey for neurovirulence testing of dengue-1 vaccine intended for use in humans. © 1987.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1016/0166-0934(87)90106-6