Yudthana Samungยุทธนา สามังKaewmala Palakulแก้วมาลา ปาละกูลChamnarn Apiwathnasornชำนาญ อภิวัฒนศรSamrerng Prummongkolสำเริง พรหมมงคลSomjai Leemingsawatสมใจ ลี้มิ่งสวัสดิ์Mahidol University. Faculty of Tropical Medicine. Department of Medical Entomology2016-04-182021-09-022016-04-182021-09-022016-04-182004https://repository.li.mahidol.ac.th/handle/20.500.14594/63432Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.244.The present study records the first successful colonization of Mansonia annulata and describes colony maintenance with modification of rearing medium and host plants. Three species of Mansonia mosquitoes (Ma. uniformis, Ma. indiana and Ma annulifera) were successfully reared in ambient environments with adult emergence rates over 50%, while Ma. bonneae and Ma. dives yielded emergence rates over 30%. Colonization of Ma. annulata was modified and improved so that they were successfully raised to adult with emergence rates of 23%. Tube sedge, Lepironia articulata, was utilized as a host plant and peat swamp water was used as a rearing medium. Yeast and small lizard droppings were added daily to the larval medium to maintain microorganisms and pH in the infusion. However, identifying suitable culture medium remains an obstacle to establishing colonies of Ma. annulata, as the culture medium is difficult to mimic in the laboratory. Further study, focusing particularly on larval attachment substrates and rearing medium, is needed to develop a standardized and practical rearing technique for Mansonia mosquitoes.engMahidol Universitylizard droppingsLepironia articulataMansoniaProceeding Poster