Chutima ThepparitSarawut KhongwichitKunjimas KetsuwanSirikwan LibsittikulPrasert AuewarakulDuncan R. SmithMahidol UniversityFaculty of Medicine, Siriraj Hospital, Mahidol University2020-01-272020-01-272019-02-01Virus Research. Vol.261, (2019), 65-7118727492016817022-s2.0-85059332604https://repository.li.mahidol.ac.th/handle/20.500.14594/50265© 2018 Elsevier B.V. The endosomal sorting complexes required for transport (ESCRT) pathway accessory protein apoptosis linked gene-2-interacting protein X (ALIX) has been shown to be upregulated during dengue virus (DENV) replication. Yeast-two-hybrid screens have additionally shown that ALIX interacts with DENV NS3 protein, but evaluation of the interaction through a replicon assay failed to show a functional significance to the interaction. In this study the interaction between DENV NS3 and ALIX was investigated by co-immunoprecipitation, and functional significance assessed by investigation of DENV production in ALIX expression regulated cells. The results showed that ALIX both interacted and co-localized with DENV NS3 protein and that upregulation of ALIX resulted in a significantly increased viral titer, while either siRNA or CRISPR-Cas9 mediated down regulation of ALIX significantly reduced viral production, without affecting relative DENV genome levels. These results are consistent with ALIX playing a significant role in the DENV replication cycle either during late infection or at viral egress.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.virusres.2018.12.015