Nale J.Y.Ahmed B.Haigh R.Shan J.Phothaworn P.Thiennimitr P.Garcia A.Abuoun M.Anjum M.F.Korbsrisate S.Galyov E.E.Malik D.J.Clokie M.R.J.Mahidol University2023-05-232023-05-232023-03-01PHAGE: Therapy, Applications, and Research Vol.4 No.1 (2023) , 11-2526416530https://repository.li.mahidol.ac.th/handle/123456789/82647We examined the activity of phages to control the growth of chicken and swine Salmonella strains in avian (CHIC-8E11), porcine (IPEC-1), and human (HT-29) cell cultures. We optimized a six-phage cocktail by selecting the five most effective myoviruses and a siphovirus that have optimal lysis on prevalent serovars. We observed ∼20% of 7 log10 PFU/well phage and 3-6 log10 CFU bacterial adhesions, and 3-5 log10 CFU bacterial invasion per 2 cm2 of the cultured cells at 2 h post-treatment. The invasive bacteria when plated had a variable reduced susceptibility to the phages. After phage application at an MOI of 10, the prophylaxis regimen had better efficacy at controlling bacterial growth with an up to 6 log10 CFU/well reduction as compared with the 1-2 log10 CFU/well bacterial reduction observed in the remedial and coinfection regimens. Our data support the development of these phages to control salmonellosis in chickens, pigs, and humans.Biochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1089/phage.2023.00012-s2.0-8515151196226416549