Nualsri C.Abdul P.M.Imai T.Reungsang A.Sittijunda S.Mahidol University2024-02-082024-02-082024-01-01Molecular Biotechnology (2024)10736085https://repository.li.mahidol.ac.th/handle/123456789/95735This study aimed to evaluate the two-stage and one-stage anaerobic co-digestion of vinasse and spent brewer yeast cells (SBY) for biohydrogen and methane production. Optimization of the vinasse-to-SBY ratio and fly ash concentration of the two-stage and one-stage production processes was investigated. In the two-stage process, the vinasse-to-SBY ratio and fly ash concentration were optimized, and the leftover effluent was used for methane production. The optimum conditions for biohydrogen production were a vinasse-to-SBY ratio of 7:3% v/w and fly ash concentration of 0.4% w/v, in which the maximum hydrogen yield was 43.7 ml-H2/g-VSadded. In contrast, a vinasse-to-SBY ratio of 10:0% v/w and fly ash concentration of 0.2% w/v were considered optimal for methane production, and resulted in a maximum methane yield of 214.6 ml-CH4/g-VSadded. For the one-stage process, a vinasse-to-SBY ratio of 10:0% v/w and fly ash concentration of 0.1% w/v were considered optimal, and resulted in a maximum methane yield of 243.6 ml-CH4/g-VSadded. In the two-stage process, the energy yield from hydrogen (0.05–0.47 kJ/g-VSadded) was 0.62%–11.78%, and the major fraction was approximately 88.22%–99.38% gain from methane (3.19–7.73 kJ/g-VSadded). For the one-stage process, the total energy yield distribution ranged from 4.20 to 8.77 kJ/g-VSadded.Chemical EngineeringBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1007/s12033-023-01015-32-s2.0-8518242871515590305