S. SirisinhaT. TengchaisriS. BoonpucknavigN. PremprachaS. RatanarapeeA. PausawasdiMahidol University2018-08-102018-08-101991-12-01Asian Pacific Journal of Allergy and Immunology. Vol.9, No.2 (1991), 153-1570125877X2-s2.0-0026338008https://repository.li.mahidol.ac.th/handle/123456789/22076A new human cholangiocarcinoma cell line (HuCCA-1) was established from cholangiocarcinoma (CCA) tissue fragments surgically removed from a Thai patient with intrahepatic bile duct cancer. The growth medium used for the primary cell culture was Ham's F12 supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml epithelial growth factor (EGF). Approximately one month later, the cells were subcultured in Ham's F12 supplemented with only 10% FBS. The population doubling time was approximately 55 hr. Staining of the cells for cytokeratin and mucin confirmed that the cells were mucin-secreting tumor of epithelial cell origin. The supernatant fluid secreted a number of non-specific tumor markers including CA 125 and traces of MCA and AFP. The ability of the HuCCA-1 cell line to synthesize specific marker that may have potential in the diagnosis of cholangiocarcinoma is now being investigated.Mahidol UniversityMedicineArticleSCOPUS