Paweorn AngsutararuxSudjit LuanpitpongPimjai ChingsuwanroteKantpitchar SupraditapornSupaporn WaeteekulPapussorn TerbtoChanchao LorthongpanichChuti LaowtammathronYaowalak U-PratyaSurapol IssaragrisilFaculty of Medicine, Siriraj Hospital, Mahidol UniversityWattanosoth Hospital2020-01-272020-01-272019-12-01Stem Cell Research. Vol.41, (2019)18767753187350612-s2.0-85074154497https://repository.li.mahidol.ac.th/handle/123456789/50006© 2019 The Author(s) Human induced pluripotent stem cells (hiPSCs) derived from dermal fibroblasts having wild type (WT) SCN5A were engineered by CRISPR/Cas9-mediated genome editing to harbor a specific point mutation (C2204>T) in SCN5A, which results in a substitution of the WT alanine by valine at codon 735 (A735V). The established MUSli009-A-1 hiPSC line has a homozygous C2204>T mutation on exon 14 of SCN5A that was confirmed by DNA sequencing analysis. The cells exhibited normal karyotype, expressed pluripotent markers and retained its capability to differentiate into three germ layers. The cardiomyocytes derived from this line would be a useful model for investigating cardiac channelopathy.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.scr.2019.101618