Witchuda SaengsawangMark M. RasenickMahidol UniversityUniversity of Illinois at ChicagoJesse Brown VA Medical Center2018-10-192018-10-192013-08-27Methods in Cell Biology. Vol.115, (2013), 173-1890091679X2-s2.0-84882632523https://repository.li.mahidol.ac.th/handle/20.500.14594/31238Microtubules, major components of the cytoskeleton, play important roles in a variety of cellular functions including mitosis, intracellular transport, and the modulation of cell morphology. Several studies have demonstrated that specific G-protein alpha subunits bind to tubulin with a high affinity (~130. nM) and elicit various functional effects on tubulin and microtubules. In this chapter, we present a description of the protocols for several methods that are used to determine the interaction between heterotrimeric G proteins and tubulin, as well as functional consequences of the interactions including protocols for protein purification, binding assays, tubulin GTPase assays, microtubule dynamics assays, and assays for cytoskeletal consequences of G-protein-coupled receptor signaling. © 2013 Elsevier Inc.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/B978-0-12-407757-7.00012-8