Le Thanh DienNguyen Vu LinhPattiya SangpoSaengchan SenapinSophie St-HilaireChannarong RodkhumHa Thanh DongSuan Sunandha Rajabhat UniversityChulalongkorn UniversityCity University of Hong KongMahidol UniversityThailand National Center for Genetic Engineering and BiotechnologyTien Giang University2022-08-042022-08-042021-09-01Journal of Fish Diseases. Vol.44, No.9 (2021), 1435-144713652761014077752-s2.0-85107405667https://repository.li.mahidol.ac.th/handle/20.500.14594/75596A rapid increase in multi-drug-resistant (MDR) bacteria in aquaculture highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB-O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB-O3 exposures caused alterations to the gills of exposed fish. Here, we set up a modified recirculation system (MRS) assembled with an NB-O3 device (MRS-NB-O3) to investigate whether MRS-NB-O3 (a) were safe for tilapia (Oreochromis niloticus), (b) were effective at reducing bacterial load in rearing water and (c) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila. The results showed no behavioural abnormalities or mortality of Nile tilapia during the 14-day study using the MRS-NB-O3 system. In the immersion challenge, although high bacterial concentration (~2 × 107 CFU/ml) was used, multiple NB-O3 treatments in the first two days reduced the bacteria between 15.9% and 35.6% of bacterial load in water, while bacterial concentration increased from 13.1% to 27.9% in the untreated control. There was slight up-regulation of non-specific immune-related genes in the gills of the fish receiving NB-O3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percentage survival (RPS) of 64.7% – 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila. In summary, the result suggests that NB-O3 is a promising non-antibiotic approach to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).Mahidol UniversityAgricultural and Biological SciencesVeterinaryArticleSCOPUS10.1111/jfd.13451