Chalermporn OngvarrasoponeYaowaluck RoshormSuthasinee SomyongChetsada PothiratanaSoontaree PetchdeeJarasporn TangkhabuanbutraSamaisukh SophasanSakol PanyimMahidol University2018-08-202018-08-202006-07-01Biochimica et Biophysica Acta - Gene Structure and Expression. Vol.1759, No.7 (2006), 328-339016747812-s2.0-33748484232https://repository.li.mahidol.ac.th/handle/20.500.14594/23009Serotonin (5-HT) mediates a number of diverse physiological functions in crustaceans by interacting with various 5-HT receptor subtypes. A putative 5-HT receptor cloned from the ovary of the black tiger prawn (Penaeus monodon) consisted of 2291 nucleotides, encoding a putative 5-HT1Pemreceptor protein of 591 amino acids. Transient expression of 5-HT1Pemin HEK293 cells demonstrated a saturable [3H]-5-HT binding with a Kd of 10.43 ± 1.13 nM and Bmaxof 1.53 ± 0.06 pmol/mg. The putative 5-HT1Pemreceptor is expressed in all tissues examined and is constitutively expressed in the ovary during ovarian maturation and spent phase. Polyclonal antibodies against the third intracellular loop (i3 loop) of the 5-HT receptor showed that the 5-HT1Pemreceptor protein was expressed in the trabeculae of ovarian stages 1 and 2 but on the cortical rod and surrounding the oocyte membrane of stages 3 and 4, suggesting that receptor localization plays a critical role in regulating ovarian maturation and spawning in penaeus shrimp. © 2006 Elsevier B.V. All rights reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.bbaexp.2006.07.004