Duangrat InthornYukinori TaniJianing ChangHirotaka NaitouNaoyuki MiyataMahidol UniversityCenter of Excellence on Environmental Health and ToxicologyUniversity of ShizuokaAkita Prefectural University2018-11-092018-11-092014-01-01Journal of Environmental Chemical Engineering. Vol.2, No.3 (2014), 1635-1641221334372-s2.0-84905457475https://repository.li.mahidol.ac.th/handle/20.500.14594/33600Environmental contamination from metals involved in industrial processes has become a serious concern, and new methods are needed to prevent their release and accumulation. In this study, we investigated the efficiencies of magnetically modified biogenic manganese oxides (MMBMOs) for simultaneously sequestering Cd2+, Co2+, Ni2+, and Co2+in the presence and absence of extra Mn2+. A Mn(II) oxidizing fungus, Acremonium strictum strain KR21-2, was employed to easily generate stable aggregations of BMO, fungal hyphae and a commercial available magnetite powder. A majority of the MMBMO aggregates (>99%) was magnetically recovered when formed with 55 mg/L Mn(II) and ≥50 mg/L magnetite powder. MMBMOs newly formed effectively sequestered heavy metal ions with high efficiency (>97%) upon repeated treatments and were subsequently almost completely trapped (>96%) using applied magnetic fields. Extra Mn2+added to these mixtures was readily oxidized to Mn oxide, providing additional sorption sites and resulting in higher sequestration efficiency compared no extra Mn2+. Inactivating the MMBMO-associated Mn(II) oxidase by heating MMBMOs at 85 °C for 1 h resulted in lower sequestration efficiency. The results presented here demonstrate that enzymatically active MMBMOs are potentially useful biogenic materials for remediating and recovering heavy metal ions from wastewater at ∼mg/L levels. © 2014 Elsevier Ltd.Mahidol UniversityChemical EngineeringEnvironmental ScienceArticleSCOPUS10.1016/j.jece.2014.06.008