Nattisa NiyomthamNuttapon ApiratikulKanoknetr SuksenPraneet OpanasopitBoon Ek YingyongnarongkulRamkhamhaeng UniversitySiam UniversityMahidol UniversitySilpakorn University2018-11-232018-11-232015-02-01Bioorganic and Medicinal Chemistry Letters. Vol.25, No.3 (2015), 496-503146434050960894X2-s2.0-84920767671https://repository.li.mahidol.ac.th/handle/20.500.14594/35504© 2015 Published by Elsevier Ltd. Twelve spermine-based cationic lipids with four different central core structures (di(oxyethyl)amino, di(oxyethyl)amino carboxy, 3-amino-1,2-dioxypropyl and 2-amino-1,3-dioxypropyl) and three hydrophobic tails (lauric acid, myristic acid and palmitic acid) were synthesized. The liposomes containing lipids and DOPE showed moderate to good in vitro DNA delivery into HeLa cells. GFP expression experiments revealed that liposomes composed of lipids with 3-amino-1,2-dioxypropyl as a central core structure exhibited highest transfection efficiency under serum-free condition. Whereas, lipid with 2-amino-1,3-dioxypropyl core structure showed highest transfection under 10% serum condition. Moreover, the liposomes and lipoplexes composted of these cationic lipids exhibited low cytotoxicity.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryMedicineArticleSCOPUS10.1016/j.bmcl.2014.12.043