Phisit KhemawootNatthaphon HunsakunachaiTosapol AnukunwithayaKunan BangphumiBoonsri OngpipattanakulWeena JiratchariyakulRuedee SoawakonthaThanakorn InthachartThaweephol Dechatiwongse Na AyudhyaSittichai KoontongkaewOrapan PoachanukoonChulalongkorn UniversityMahidol UniversityHuachiew Chalermprakiet UniversityThammasat UniversityFaculty of Medicine, Thammasat University2018-12-112019-03-142018-12-112019-03-142016-08-01Planta Medica. Vol.82, No.13 (2016), 1186-119114390221003209432-s2.0-84992303032https://repository.li.mahidol.ac.th/handle/20.500.14594/42939© Georg Thieme Verlag KG Stuttgart New York. Rhizomes of Zingiber cassumunar have been used for many years in traditional Thai medicine as an anti-inflammatory agent. The major bioactive component of this plant is Compound D [E-4-(3′, 4′-dimethoxyphenyl)but-3-en-1-ol], which is a strong smooth muscle relaxant, and has antihistamine and anti-inflammatory actions. There is, however, incomplete information available for the pharmacokinetics of Compound D in mammals. In this study, we examined the pharmacokinetic profiles of Compound D in male Wistar rats. A standardized extract of Z. cassumunar containing 4% w/w Compound D was administered intravenously at 25 mg/kg or by oral gavage at 25, 75, or 250 mg/kg to Wistar rats. Blood, tissues, urine, and feces were collected from 0 to 48 h after dosing and the level of Compound D was determined by liquid chromatography-tandem mass spectrometry. The concentration of Compound D ranged from 10-100 μg/L, reached a maximum approximately 0.15 h after oral dosing. Compound D exhibited an excellent tissue to plasma ratio, ranging from 1- to 1000 in several organs at 1-4 h after oral dosing. Less than 1% of unchanged Compound D was excreted in the urine and feces. Further studies on tissue uptake and metabolite identification are required to obtain complete pharmacokinetic information and to develop appropriate dosing strategies of Compound D and the standardized extract of Z. cassumunar.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryMedicineArticleSCOPUS10.1055/s-0042-104658