Somjit ChaiwattanarungruengpaisanMetawee ThongdeeSongtham AnuntakarunSunchai PayungpornNlin AryaApichart PunchukrangPongrama RamasootaSombat SinghakaewThassanant AtithepLadawan SariyaFaculty of Tropical Medicine, Mahidol UniversitySongkhla Rajabhat UniversityVidyasirimedhi Institute of Science and TechnologyChulalongkorn UniversityMahidol University2022-08-042022-08-042021-05-01PLoS ONE. Vol.16, No.5 May (2021)193262032-s2.0-85106666747https://repository.li.mahidol.ac.th/handle/20.500.14594/79357Chlamydia is a known pathogen in both saltwater and freshwater crocodiles. However, the exact species/strain has not been clearly identified. In this study, we successfully cultivated Siamese crocodile Chlamydia in McCoy cells at a temperature of 30◦C. Electron microscopy; phylogeny based on nine conserved taxonomically informative markers, on ompA, or on seven housekeeping genes; and whole-genome sequencing and analysis of the isolate confirmed the identity of the isolate as a new member of the genus Chlamydia, a new species that we name Chlamydia crocodili.Mahidol UniversityMultidisciplinaryArticleSCOPUS10.1371/journal.pone.0252081