Haruna L.A.Purnama L.O.M.J.Fristiohady A.Masrinoul P.Komaikul J.Ketsuwan K.Asasutjarit R.Mahidol University2025-06-052025-06-052025-04-01Indonesian Journal of Science and Technology Vol.10 No.1 (2025) , 171-19025281410https://repository.li.mahidol.ac.th/handle/123456789/110464Herpes simplex virus (HSV)-1-induced retinitis is one of ophthalmic viral infections. It is found in some immunocompromised and elderly patients and is a cause of complete blindness. Nowadays, an intravitreal (IVT) injection of ganciclovir (GCV) is applied to treat this disease. Unfortunately, frequent IVT injections induce adverse effects in the eye. GCV-loaded chitosan nanoparticles (GCV-CS-NPs) were developed to reduce the number of IVT injections. This study developed GCV-CS-NPs and investigated their activity against HSV-1. GCV-CS-NPs were prepared using an ionic gelation method with varying formulation compositions. The particle size, polydispersity index (PDI), zeta potential, pH, drug release, and activity against the HSV-1 were investigated. The results showed GCV-CS-NPs with particle sizes of around 161 to 294 nm, moderate PDI values, and zeta potentials of between +8.0 and +18.1 mV. These properties strongly depended on the formulation compositions. The release rate of GCV from the optimized GCV-CS-NPs (3C-0.5G-1T-4.5) in the simulated vitreous humor regarding Higuchi’s model was 4.89±0.48 µg/min1/2. The IC50 against the HSV-1 was 25.12±0.02 µg/mL. 3C-0.5G-1T-4.5 could be accepted as a promising delivery system of GCV to the vitreous humor via IVT injection and had a potential for being subjected to further in vivo studies.Earth and Planetary SciencesChemical EngineeringComputer ScienceEngineeringArticleSCOPUS10.17509/ijost.v10i1.809982-s2.0-10500653192025278045