Porntida KobpornchaiEllen Alana TiffneyPoom AdisakwattanaRobin J. FlynnFaculty of Tropical Medicine, Mahidol UniversityUniversity of Liverpool2022-08-042022-08-042021-04-01Cellular Immunology. Vol.362, (2021)10902163000887492-s2.0-85101338588https://repository.li.mahidol.ac.th/handle/20.500.14594/77305We have previously identified a cystatin, TsCstN, derived from the L1 stage of Trichinella spiralis and have shown that this protein is internalised in macrophages. Here we sought to address if this macrophage-TsCstN interaction could alter downstream T-cell priming. Using LPS-primed macrophages to stimulate T-cells in a co-culture system with or without TsCstN we assessed the resultant T-cell outcomes. IFN-γ, both protein and mRNA, but not IL-17A was negatively regulated by inclusion of TsCstN during macrophage priming. We identified a cell–cell contact independent change in the levels of IL-12 that led to altered phosphorylated STAT4 levels and translocation. TsCstN also negatively regulated the autonomous response in the myotubule cell line, C2C12. This work identifies a potential pathyway for L1 larvae to evade protective Th1 based immune responses and establish muscle-stage T. spiralis infection.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1016/j.cellimm.2021.104303