Jean StanleyViroje ChongkolwatanaPham Tuan DuongPimpun KitpokaSusan L. StramerNguyen Thi Thanh DungKacie E. GrimmAnyarin PojanasingchodPanitita SuksomboonvongSusan A. GalelAmerican Red CrossFaculty of Medicine, Ramathibodi Hospital, Mahidol UniversityFaculty of Medicine, Siriraj Hospital, Mahidol UniversityMedical and Scientific AffairsNational Institute of Hematology and Blood Transfusion2020-11-182020-11-182020-01-01Transfusion. (2020)15372995004111322-s2.0-85092146575https://repository.li.mahidol.ac.th/handle/20.500.14594/59999© 2020 The Authors. Transfusion published by Wiley Periodicals LLC. on behalf of AABB. Background: Chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses are of concern due to the potential of transfusion transmission in blood, especially in regions such as Southeast Asia where the viruses are endemic. The recent availability of nucleic acid testing (NAT) to screen blood donations on an automated platform provides the opportunity to detect potentially infectious units in asymptomatic donors. Study Design and Methods: Three thousand blood donations from Vietnam and 6000 from Thailand were screened with a real-time polymerase chain reaction (PCR) test (cobas CHIKV/DENV, Roche Diagnostics, Indianapolis, IN) and equal numbers on cobas Zika (Roche Diagnostics). Reactive samples were tested by alternative NAT with resolution of discordant results by heminested PCR. Throughput of simultaneous testing of the two assays on the cobas 8800 system (Roche Diagnostics) was evaluated. Results: In Vietnam, 9 of 3045 samples were reactive for DENV and all were confirmed, for a prevalence (with 95% confidence interval [CI]) of 0.296% (0.135-0.560). In Thailand, 2 of 6000 samples were reactive for CHIKV, 4 of 6000 for DENV, and 1 of 6005 for ZIKV, and all confirmed. The prevalence of CHIKV is 0.033% (0.004-0.120), DENV 0.067% (0.018-0.171), and ZIKV 0.017% (0.000-0.093). The overall specificity for the cobas CHIKV/DENV and cobas Zika tests was 100% (99.959-100). For the simultaneous assay testing, 960 test results were available in 7 hours and 53 minutes. Conclusion: Detection of CHIKV, DENV, and ZIKV RNA in donor samples in Vietnam and Thailand indicate the presence of the virus in asymptomatic blood donors. The cobas 6800/8800 systems (Roche Molecular Systems, Pleasanton, CA) enable screening blood donations in endemic areas for these viruses together or separately.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1111/trf.16110