Supaporn HasthanasombutKanyaratt SupaibulwatanaMasahiro MiiIkuo NakamuraChiba UniversityMahidol University2018-05-032018-05-032011-01-01Plant Cell, Tissue and Organ Culture. Vol.104, No.1 (2011), 79-89016768572-s2.0-78649936030https://repository.li.mahidol.ac.th/handle/20.500.14594/11371Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB), an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings. © 2010 Springer Science+Business Media B.V.Mahidol UniversityAgricultural and Biological SciencesGenetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity toleranceArticleSCOPUS10.1007/s11240-010-9807-4