Jaroenram W.Teerapittayanon S.Suvannakad R.Pengpanich S.Kampeera J.Arunrut N.Dangtip S.Sirithammajak S.Tondee B.Khumwan P.Japakasetr S.Leaungwutiwong P.Chatnuntawech I.Kiatpathomchai W.Mahidol University2024-08-092024-08-092024-10-01Diagnostic Microbiology and Infectious Disease Vol.110 No.2 (2024)07328893https://repository.li.mahidol.ac.th/handle/20.500.14594/100396COVID-19 has afflicted millions of lives worldwide. Although there are many rapid methods to detect it based on colorimetric loop-mediated isothermal amplification, there remains room for improvement. This study aims to 1) integrate multiple primers into a singleplex assay to enhance the diagnostic sensitivity, and 2) utilize a high-throughput smartphone-operatable AI-driven color reading tool to enable a rapid result analysis. This setup can improve the sensitivity by 10–100 times and can analyze approximately 6700 samples per minute. The assay is simpler than RT-qPCR, with a turnaround time of less than 75 min. It can detect various types of SARS-CoV-2 by targeting 3 genes, increasing the likelihood that it will remain effective even if the virus undergoes mutations in any single target gene. In summary, it affords potential for adaptation to detection of new/re-emerging diseases with the visual readout for maximum assay simplicity and AI-operated mode for large-scale testing.MedicineArticleSCOPUS10.1016/j.diagmicrobio.2024.1164462-s2.0-8520022179118790070