Piyawat PitikulthamChakrit SriprachuabwongPornpimol SritongkhamMahidol UniversityThailand National Electronics and Computer Technology Center2018-11-232018-11-232015-01-01BMEiCON 2014 - 7th Biomedical Engineering International Conference. (2015)2-s2.0-84922978468https://repository.li.mahidol.ac.th/handle/123456789/35946© 2014 IEEE. A promising acetylcholine biosensor based on graphene-PEDOT:PSS (GR-PEDOT:PSS) and bienzyme, choline oxidase (ChOx) and acetylcholine esterase (AChE), modified electrode was successfully fabricated. GR-PEDOT:PSS ink was directly dropped on the screen printed carbon electrode (SPE), and then ChOx and AChE in the optimized loading ratio were immobilized on GR-PEDOT:PSS/SPE by the 10% glutar-aldehyde (GA) vapor cross-linked for 8 minutes. 1% Nafion was coated on the outermost layer of AChE-ChOx/GR-PEDOT:PSS/SPE for performing as a protective film. For amperometric responding, our acetylcholine biosensor showed a low limit detection, high linearity and sensitivity, which were 50 μM, 50 to 250 μM and 486.1 nA/mM, respectively, at -200 mV versus Ag/AgCl reference electrode. Moreover, this biosensor has a simple preparation, low-cost fabrication, and stable detection, thus it is a novel tool for acetylcholine determination.Mahidol UniversityEngineeringConference PaperSCOPUS10.1109/BMEiCON.2014.7017408