Publication: Variable expression of B-cell transcription factors in reactive immunoblastic proliferations: A potential mimic of classical Hodgkin lymphoma
Issued Date
2014-01-01
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ISSN
15320979
01475185
01475185
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2-s2.0-84914708594
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Surgical Pathology. Vol.38, No.12 (2014), 1655-1663
Suggested Citation
Jitsupa Treetipsatit, Lisa Rimzsa, Thomas Grogan, Roger A. Warnke, Yasodha Natkunam Variable expression of B-cell transcription factors in reactive immunoblastic proliferations: A potential mimic of classical Hodgkin lymphoma. American Journal of Surgical Pathology. Vol.38, No.12 (2014), 1655-1663. doi:10.1097/PAS.0000000000000266 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34765
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Title
Variable expression of B-cell transcription factors in reactive immunoblastic proliferations: A potential mimic of classical Hodgkin lymphoma
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Abstract
Copyright © 2014 by Lippincott Williams & Wilkins. Reactive immunoblastic proliferations can histologically mimic classical Hodgkin lymphoma (CHL), and show diffuse CD30 expression in large cells. The lack of expression of CD15 in a subset of CHL further complicates their separation from immunoblastic proliferations. Loss of expression of B-cell transcription factors is frequently exploited in making a diagnosis of CHL; however, the staining patterns of B-cell transcription factors in immunoblastic proliferations have not been extensively studied. Thirty-three cases of reactive immunoblastic proliferations were evaluated using a panel of immunohistochemistry for CD30, CD15, CD20, CD3, κ, λ, CD45RB, MUM1, PAX5, OCT2, and BOB.1, as well as Epstein-Barr virus (EBV)/EBV-encoded ribonucleic acid in situ hybridization. A newly developed dual-color chromogenic in situ hybridization technology for detection of κ/λ mRNAs was also used. The majority of immunoblasts expressed CD30 in 14 of 33 (42%) cases; none expressed CD15. Loss or weak expression of at least 1 transcription factor in B immunoblasts, most commonly PAX5, was noted in 24 of 29 (83%) cases. A polytypic light chain expression pattern was detected by immunohistochemistry in 14 of 22 (63.6%) cases and by dual-color chromogenic in situ hybridization in 9 of 10 (90%) cases studied. EBV-encoded ribonucleic acid was detected in 8 of 33 (24.2%) cases, 5 of which were clinically unrelated to infectious mononucleosis. We conclude that B-cell transcription factors can show loss or weak expression in a significant proportion of reactive immunoblastic proliferations, and, therefore, staining for B-cell transcription factors together with CD30 should be interpreted with caution before a diagnosis of CHL is made.