Mahidol University's Institutional Repository
คลังสารสนเทศสถาบันของมหาวิทยาลัยมหิดล
"Wisdom Repository You Discover"
To collect Mahidol University's academic publications and intellectual properties more than 39 faculties
To present over 50,000 items of information in digital formats
To make it easy to access to all information at anytime, anywhere
Communities in Mahidol IR
Select a community to browse its collections.
Recent Submissions
Multifactorial Fall Prevention Intervention with Tai Chi on Physical Function in Urban Community-Dwelling Older Adults with Fall History
(2026-05-01) Khruesanit S.; Kaewwilai L.; Amnatsatsue K.; Kerdmongkol P.; Khruesanit S.; Mahidol University
Background: Falls among older adults are a significant public health concern associated with reduced lower extremity muscle strength and balance. Objective: This study aimed to evaluate the effectiveness of a multifactorial fall prevention intervention with Tai Chi on lower extremity muscle strength and balance among community-dwelling older adults with a fall history in Bangkok, Thailand. Methods: A quasi-experimental, two-group pretest–posttest design was employed (n = 60; experimental = 30, comparison = 30). The experimental group received an 8-week program incorporating fall prevention education and fall risk assessment, Tai Chi training, scenario-based risk management, home hazard assessment, digital reinforcement via LINE Official Account, and family-engaged home visits. The comparison group received usual care and a fall prevention manual. Results: Post-inter-vention, the experimental group demonstrated a statistically significant improvement in lower extremity muscle strength compared with the comparison group. The mean Five Times Sit-to-Stand Test (FTSST) completion time decreased from 11.64 ± 2.07 seconds to 9.63 ± 1.53 seconds in the experimental group, whereas only a minimal change was observed in the comparison group (15.08 ± 3.40 seconds to 15.74 ± 3.75 seconds). ANCOVA-adjusted means confirmed a significant between-group difference in lower extremity function, with the experimental group demonstrating a shorter FTSST completion time than the comparison group (11.04 vs. 14.20 seconds; F (1,57) = 36.33, p < .001, partial η² = .389). Dynamic balance also improved significantly in the experimental group compared with the comparison group. Dynamic balance improved significantly following the intervention. The mean Timed Up and Go (TUG) test time decreased from 10.45 ± 1.86 to 8.75 ± 1.55 seconds in the experimental group, compared with a marginal reduction from 14.13 ± 3.95 to 13.93 ± 4.08 seconds in the comparison group. ANCOVA-adjusted mean TUG times demonstrated a significant between-group difference, favoring the experimental group (10.34 vs. 12.34 seconds; F(1,57) = 17.53, p < .001, partial η² = .235). Static balance also showed significant improvement. Within the experimental group, Wilcoxon signed-rank test results indicated a significant increase in static balance performance (pre-intervention: median = 40.00 s, IQR = 7.75; post-intervention: median = 40.00 s, IQR = 0.00; Z =-2.437, p = .015). Furthermore, post-intervention static balance was significantly better in the experimental group than in the comparison group, as demonstrated by the Mann– Whitney U test (Z =-2.762, p = .006). Conclusion: This multifactorial program incorporating Tai Chi significantly improved lower extremity muscle strength and balance, providing a feasible, low-cost model to improve physical function and reduce fall risk among community-dwelling older adults.
Epigallocatechin-3-gallate, l-theanine and theophylline abolish high-glucose-induced renal cell senescence and subsequent epithelial-mesenchymal transition and fibroblast activation via SIRT1/PGC-1α axis: Implications for diabetic kidney disease
(2026-09-01) Kanlaya R.; Yoodee S.; Nonthawong K.; Subkod C.; Nanthawuttiphan S.; Thongboonkerd V.; Kanlaya R.; Mahidol University
Recent research has highlighted the converging step of diabetic kidney disease (DKD) and chronic kidney disease (CKD) at cellular senescence and fibrotic processes. Therefore, therapeutic interventions targeting cellular senescence may ameliorate or slow the progression of DKD and CKD. This study evaluated the anti-senescence properties of the three most abundant phytochemicals in green tea, including epigallocatechin-3-gallate (EGCG), l -theanine and theophylline, in proximal tubular epithelial cells (PTECs) exposed to high-glucose. Additionally, their anti-fibrotic activities were examined in distal tubular epithelial cells (DTECs) and renal fibroblasts treated with the conditioned medium containing senescence-associated secretory phenotypes (SASPs) secreted from high-glucose-treated PTECs. The results showed that high-glucose triggered various senescence markers in PTECs, including cell enlargement, increased senescence-associated β-galactosidase activity, increased p21 level, S-phase cell-cycle arrest, suppressed cellular metabolic activity, and elevated SASPs levels. While phosphorylated (p)-p38, p-glycogen synthase kinase-3β (p-GSK-3β) and β-catenin remained unchanged, sirtuin-1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) were suppressed by high-glucose. Moreover, the conditioned medium containing SASPs secreted from high-glucose-treated PTECs induced epithelial-mesenchymal transition (EMT) (shown by decreased E-cadherin and zonula occludens-1 (ZO-1), and increased vimentin and fibronectin) in DTECs and renal fibroblast activation (shown by increased fibronectin, collagen 1α, vimentin and actin stress fiber). However, EGCG, l -theanine and theophylline effectively abolished all these high-glucose-induced effects on senescence and SIRT1/PGC-1α axis in PTECs, EMT in DTECs, and renal fibroblast activation. In summary, this study highlights the anti-senescence, anti-EMT and anti-fibrotic properties of these green tea bioactive compounds via SIRT1/PGC-1α axis to counteract the high-glucose-induced effects in DKD.
Erectile dysfunction severity and penile Doppler findings in pelvic fracture urethral injury: A descriptive cohort study
(2026-07-01) Joshi P.M.; Ratanapornsompong W.; Ocampo M.A.; Navarro J.; Patwardhan S.S.; DeWin G.; Thiessen F.; Wood D.; Kulkarni S.B.; Joshi P.M.; Mahidol University
Introduction: Erectile dysfunction (ED) is a common and multifactorial complication after pelvic fracture urethral injury (PFUI), yet objective characterization of ED severity and penile Doppler findings remains limited. We aimed to describe ED severity categories and penile Doppler findings in men with PFUI undergoing anastomotic urethroplasty. Methods: We performed a retrospective analysis of a prospectively maintained database. Among 110 men who underwent anastomotic urethroplasty for PFUI between September 2022 and September 2023, 97 with complete preoperative penile Doppler ultrasound and standardized Sexual Health Inventory for Men assessment were included. Pelvic fractures were classified using the Young–Burgess system. All patients received tadalafil 5 mg daily for 6 months. Postoperative Doppler was not performed. Results: The median age was 29.5 years with a median follow-up of 11 months. Urethroplasty success rate was 85%(83/97). Preoperatively, 56 out of 97 patients had ED (42 mild/moderate; 14 severe). Penile Doppler abnormalities were nearly universal: 93 had cavernosal, and 83 had dorsal artery insufficiency. Postoperatively, 13 out of 41 men developed de novo ED, while 13 out of 56 improved by at least one severity category. Among 76 patients with complete fracture data, greater fracture severity was associated with worse postoperative ED, and worsening erectile function was more frequent among patients with urethroplasty failure. Conclusions: ED and penile Doppler abnormalities are highly prevalent after PFUI. ED severity correlated with pelvic injury severity, supporting the concept that ED reflects global trauma burden rather than isolated vascular pathology. Given the retrospective design and routine postoperative use of PDE5 inhibitors, these findings should be considered descriptive and hypothesis-generating.
Comparison of two algorithms of APTT-based lupus anticoagulant assay, two Dilute Russell viper venom time reagents, and silica clotting time
(2026-01-01) Wongkrajang P.; Pientong T.; Hanyongyuth R.; Tientadakul P.; Wongkrajang P.; Mahidol University
Lupus anticoagulants (LA) are heterogeneous antiphospholipid antibodies that interfere with phospholipid-dependent coagulation assays, resulting in considerable variability among detection methods. Although international guidelines recommend a stepwise approach incorporating screening, mixing, and confirmatory testing, integrated strategies omitting routine mixing studies are widely used. Different percentile-based cutoffs have been proposed for defining LA positivity.We retrospectively analyzed 135 citrated plasma samples requested for LA testing. Five LA detection approaches were evaluated: 4 integrated assays and 1 activated partial thromboplastin time (APTT)-based approach following the ISTH-recommended stepwise algorithm with a mixing study. The integrated assays comprised silica clotting time, dilute Russell viper venom time (dRVVT) using 2 different reagent systems, and an APTT-based assay. Precision studies and reference intervals were established, and LA positivity rates were compared using 97.5th and 99th percentile cutoffs. Inter-assay agreement and associations with anticardiolipin (aCL) and anti-β2-glycoprotein I (aβ2GPI) antiphospholipid antibodies were assessed. LA positivity rates varied across procedures (19.3%-35.6%) at the 97.5th percentile. Application of the 99th percentile decreased positivity for dRVVT-HemosIL and APTT-based assays. Positivity increased at the 97.5th percentile in patients tested according to ISTH indications, with minimal impact in noncompliant cases. Inter-assay agreement ranged from fair to substantial and was influenced by assay type and cutoff definition. APTT-based assays showed the strongest associations with aCL and aβ2GPI antibodies. LA detection is strongly influenced by assay selection and cutoff strategy. Positivity rate-based evaluation provides a practical framework for comparing LA assays in laboratory practice, particularly in the absence of a reference standard.
Measurement of Plasma Free Hemoglobin Using the Hemolysis Index of the Mindray BS-800
(2026-01-01) Viruttanachai P.; Chanpo N.; Thedsawad A.; Kittivorapart J.; Ruchutrakool T.; Hantaweepant C.; Viruttanachai P.; Mahidol University
Background: The Mindray BS-800 chemistry analyzer (Mindray Bio-Medical Electronics) has a hemolysis index (HI) function and is a candidate method for detecting free hemoglobin (fHb). This study aimed to validate the HI on the Mindray BS-800 for measuring plasma fHb. Methods: Using EDTA plasma diluted with normal saline, the analyzer measured hemoglobin absorbance at 570 and 605 nm. Results were compared with those of the Harboe method (reference standard), which measures absorbance at 380, 415, and 450 nm. Precision, linearity, limit of detection, interference, reference range, and carryover were also assessed. Results: Replication experiments showed within-run and between-run precision of 0.41%–1.89% and 0.71%–2.10%, respectively. Linearity of fHb measurements was established over 2.3–507.9 mg/dL. The limits of detection and quantitation were 2 and 5 mg/dL, with coefficient of variation values of 16.28% and 6.37%, respectively. No notable interference was observed at triglyceride concentrations up to 520.9 mg/dL; however, bilirubin levels of 14.2–34.7 mg/dL produced a negative bias of up to 15%. The reference range was established at ≤ 23.9 mg/dL. Within the 0–130 mg/dL range, fHb correlated strongly with the Harboe method, yielding the regression equation: HI (mg/dL) = 1.0886 × Harboe (mg/dL) − 0.3071 (r = 0.9967). Conclusions: Measurement of fHb using the HI on the Mindray BS-800 analyzer provides accurate and reliable results. This approach offers a practical alternative to the Harboe method for routine clinical laboratory use, with improved safety and turnaround time.