Mahidol University's Institutional Repository
คลังสารสนเทศสถาบันของมหาวิทยาลัยมหิดล
"Wisdom Repository You Discover"
To collect Mahidol University's academic publications and intellectual properties more than 39 faculties
To present over 50,000 items of information in digital formats
To make it easy to access to all information at anytime, anywhere
Communities in Mahidol IR
Select a community to browse its collections.
Recent Submissions
RSjournal Vol. 5 No. 1-2
(2552)
Modification and Characterization of Waxy and Native Tapioca Starches for Stabilizing Resveratrol Pickering Emulsion
(2025-12-18) Wannarat R.; Pleanklay A.; Boonsith S.; Thepwatee S.; Wannarat R.; Mahidol University
Tapioca starch is a promising biopolymer for use as a solid particle stabilizer in Pickering emulsions. This study aimed to enhance the emulsifying performance of native (NS) and waxy (WS) tapioca starches through antisolvent precipitation followed by esterification with 3% (w/v) octenyl succinic anhydride (OSA). Structural and functional changes were characterized by SEM, XRD, DSC, FTIR, contact angle measurements, and degree of substitution analysis. The modifications transformed the starch morphology from smooth granules into rough, aggregated particles and reduced crystallinity, gelatinization enthalpy, and the intensity of characteristic functional groups. Both starch types exhibited similar structural changes; however, the modified native starch (NSp-OSA) showed higher hydrophobicity (contact angle: 103.73 ± 0.50°) than the modified waxy starch (WSp-OSA, 84.83 ± 0.60°), resulting in improved emulsion stability. Emulsion screening using jojoba oil identified optimal starch-to-oil ratios of 2.0:1.2 g/g for NSp-OSA and 1.0:1.2 g/g for WSp-OSA. These conditions were then applied for encapsulating resveratrol (RES). Due to the poor solubility of RES in oil, a ternary solvent system comprising of PEG 400, jojoba oil, and olive oil (0.1:0.5:0.5:0.5 g/g) was formulated to enhance solubility and emulsion uniformity. The NSp-OSA formulation produced more stable RES-loaded emulsions than WSp-OSA. This study demonstrates the potential of modified tapioca starches as bio-based stabilizers for emulsion-based delivery systems.
Dermatomyositis assessment of rash via telemedicine: a preliminary study
(2026-02-01) Pongtarakulpanit N.; Bishnoi A.; Ascherman D.P.; Moghadam-Kia S.; Oddis C.V.; Aggarwal R.; Pongtarakulpanit N.; Mahidol University
Web-Based Application for Cognitive and Functional Assessments in Dementia Screening: Mixed Methods, User-Centered Development Approach
(2026-01-01) Piyaamornpan N.; Srisuwannanukorn S.; Tangthamrongthanawat K.; Mekhasingharak P.; Rattanabannakit C.; Hunnangkul S.; Wongkom N.; Senanarong V.; Piyaamornpan N.; Mahidol University
Background: Digital health technologies offer new opportunities for cognitive screening and monitoring among older adults. In Thailand, where dementia prevalence is rising, accessible web-based cognitive tools remain limited despite their potential to facilitate early detection and community-based assessment. Understanding usability and validity is critical to ensure successful implementation in real-world contexts. Objective: This study aimed to develop and validate a web-based application, Healthy Brain Test, for cognitive and functional assessments in dementia screening among older Thai adults. Specific objectives were to (1) design user-centered cognitive modules covering key cognitive domains and (2) evaluate correlations between the web-based assessments and conventional clinical tools to determine diagnostic cutoffs for cognitive impairment. Methods: We designed Healthy Brain Test as a self-administered web application suitable for older users and their caregivers. The platform includes digital versions of the Thai Mental State Examination (e-TMSE), a clock drawing test, and a category verbal fluency test, along with electronic versions of the short form of the Informant Questionnaire on Cognitive Decline in the Elderly (IQCODE-16) and cognitive instrumental activities of daily living (IADLs). Participants completed both web-based and paper-based assessments. Correlations between modalities were analyzed, and receiver operating characteristic (ROC) curves were generated to determine sensitivity and specificity. Data were analyzed using SPSS for Windows, version 30.0 (IBM Corp) and MedCalc Statistical Software (MedCalc Software Ltd). Results: A total of 198 older adults participated (women: 137/198, 69.2%; median age 69.4 years), with 57.1% (113/198) having more than 6 years of education. Of the 198 participants, 44 were diagnosed with major neurocognitive disorder, 58 were diagnosed with mild neurocognitive disorder, and 96 were cognitively normal. The e-TMSE showed strong agreement with the traditional TMSE (r=0.837; P<.001). Category verbal fluency, IQCODE-16, and IADL modules also demonstrated significant correlations (P<.001). The e-TMSE achieved an area under the ROC curve of 0.84 (bootstrapped 95% CI 0.78-0.89); a cutoff ≤23 provided 88.6% sensitivity and 70.1% specificity for identifying major neurocognitive disorder. Participants reported high ease of use and engagement during pilot testing. Conclusions: Healthy Brain Test demonstrated strong validity and usability as a web-based cognitive and functional assessment platform for dementia screening. Its integration of established cognitive measures into a digital interface enables remote, accessible, and user-friendly evaluation for older adults and caregivers. Future research should assess long-term feasibility, user adherence, and integration with clinical workflows to support large-scale screening initiatives.
Cross-Induction of Anti-Complexing Antibodies in Patients Treated with Botulinum Toxin Formulations Containing Complexing Proteins
(2026-02-14) Srinoulprasert Y.; Sirisuthivoranunt S.; Sripatumtong C.; Tansit T.; Yamlexnoi P.; Meethong O.; Wanitphakdeedecha R.; Srinoulprasert Y.; Mahidol University
Botulinum toxin type A (BoNT/A) formulations differ in their content of non-toxic accessory proteins, also known as complexing proteins (CPs), which may influence immunogenicity. Some BoNT/A products share structurally similar CPs, potentially leading to antibody cross-reactivity among formulations. This prospective study investigated whether patients treated with different BoNT/A products develop cross-reactive anti-CP antibody responses. One hundred participants were allocated into five treatment groups, each receiving a single BoNT/A formulation: incobotulinumtoxinA (IncoA), onabotulinumtoxinA (OnaA), abobotulinumtoxinA (AboA), letibotulinumtoxinA (LetiA), or prabotulinumtoxinA (PraboA). Each participant received 50 units or equivalent dosing. Serum samples were collected 180 days post-injection, and anti-CP antibodies were quantified using an absorption ELISA and compared with a toxin-naïve control group. IncoA did not induce significant anti-CP antibody responses. In contrast, higher antibody levels were observed in the OnaA, LetiA, and PraboA groups against multiple CPs, suggesting structural similarity and cross-reactivity. AboA primarily induced antibodies directed against its own CPs and those of PraboA. These findings demonstrate that CP-containing formulations can induce cross-reactive antibody responses, whereas CP-free incobotulinumtoxinA exhibits minimal immunogenicity. This study highlights the importance of CP composition in guiding clinical product selection, particularly in patients requiring repeated BoNT/A administration.