Micropropagation for conservation of Zingiberaceae in Surin province, Thailand

Abstract

© 2017, International Society for Horticultural Science. All rights reserved. The Zingiberaceae is an important species used in medicine and cosmetics. An optimized protocol was developed for conservation of endemic species of Zingiberaceae in Surin province, Thailand included Curcuma singularis Gagnep., Kaempferia galanga Linn., and Zingiber officinale Rosc. through rhizome buds using tissue culture was investigated. Sterile rhizome buds were cut and then cultured on MS agar medium (1962) supplemented with 0, 1, 2 and 3 mg L-1BA, as a plant growth regulator for shoot formation. The results showed that rhizome buds of C. singularis Gagnep. and K. galanga Linn. cultured on MS agar medium supplemented with 2 mg L-1BA gave the highest shoot formation of 4.5 and 9 shoots bud-1, respectively. The addition of 1 mg L-1BA to the MS agar medium was found to be the best for induction of shoots after culture of Z. officinale Rosc. rhizome buds (11 shoots bud-1) for 90 days of culture at 27°C under 16-h light photoperiod. Root formation in all media was observed. Regeneration of shoot and root at the same time in the same medium reduced time and cost for micropropagation. The plantlets were successfully acclimatized when transferred to outdoor for 90 days.